Protein Analysis: Luciferase Assay
AlleleBalanced Luciferase Assay Kit is a novel method for measuring the firefly (Photinus pyralis) luciferase gene (luc) expressions in mammalian tissue culture cells or in bacteria. After extensive research and development efforts, this new assay system offers extremely sensitive detection tools for those using luc as reporter. It provides a simple reaction system balancing sensitivity and stability to suit most routine Luciferase-based gene expression analysis. The kit can be used in low- or high-throughput applications at a much reduced cost. The AlleleBalanced Luciferase Assay Kit is also very easy to use, ship, and store.
Luciferase Assay Features
- Stability, offers maintenance of more than 95% signal level after 3 hours
- Easy to use, simply lyse the cells, mix, and read
- High sensitivity which produces more signal than similar leading brands
- Low cost shipping; the reagents are stable for a few days at room temperature, eliminating the need for cold packaging.
- Easy storage, -20oC is recommended for long term storage; repeated freezing and thawing will not affect its effectiveness
Protein Extraction Reagents
AlleleExtract Protein Reagents are designed to extract proteins from bacterial or mammalian cells. These reagents replace the need for other protein extraction procedures such as sonication which can be time consuming, and inefficient. AlleleExtract-B Protein Reagent provides a single-step method of gently extracting proteins from a wide range of bacterial cells. Using this reagent eliminates the need for repeated sonication and freezing/thawing steps that are commonly used to release recombinant proteins from E coli. The extracted proteins are suitable for many affinity purification and analysis methods, (e.g. GST and 6xHis purification systems). If preferred, DNAse I, lysozyme, protease inhibitor, salts, etc. may be added to the reagent. AlleleExtract-B Protein Reagent offers a several fold increase in the yield of soluble proteins when compared with other commercial lysis systems. It may also be used for preparation of inclusion bodies from total cell lysate.
AlleleExtract-M Protein Reagent provides an effective and gentle method for extracting proteins from mammalian cells. This reagent uses a non-ionic detergent in 25 mM Tris, pH 7.4, and 250mM to extract nuclear and cytoplasmic protein from cultured cell lines. AlleleExtract-M may be used conjunctively with reporter assays, immunoassays, and protein purification. It is easily digested with protein assays, such as Western blotting, Coomassie Blue, Bradford, and BCA Protein Assays.