Retrovirus is a proven method for efficient delivery of genes into dividing mammalian cells. Because the gene of interest integrates into the host cell genome, retroviral expression offers more consistency and reliability than episomal expression. In addition, Allele’s retroviral system is safe and easy to use because the viral particles produced are replication incompetent and can be easily inactivated*.
Allele’s retrovirus system includes a retroviral vector and a packaging cell line. The retroviral vector contains viral packaging signal, transcription and processing elements and your cloned gene of interest. The packaging cell line carries viral genes, i.e., gag, pol and env, essential for virus reproduction. After the retroviral vector is transfected into the packaging cell line, viral particles are produced. These viral particles acquired envelope glycoproteins residing on the packaging cell’s membrane and these glycoproteins determine which kind of cells the viral particles can infect. Allele’s Retroviral Expression System comes with both an ecotropic (capable of gene delivery into murine or rat cells) and amphotropic packaging cell line (capable of gene delivery into dividing cells of most mammalian species).
*A biosafety level 2 facility is required to work with retroviral particles in the United States. Please check your institutional policy for specific requirement.