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Allele is bringing to you some Allele-only products and some common reagents. What’s unique about Allele’s "common" enzymes? Exceptionally high quality, low price, and convenience --we are NIH grant-supported scientists and we understand your needs. Linear expression cassettes allow researchers to transfect cells on the same day that they receive the product. The results can be observed as early as the next day. No cloning or sequencing steps are required. This unique linear format is suitable for high throughput experiments, and for conjugating tracers or carriers to the cassette. The complete kit includes a p53 RNAi positive control, PCR mastermix, and transfect reagent AvantGene. Catalog Reference Amount List Price Cat # ABP-LS-1055 40 PCR rxns $ 290 Cat # ABP-LS-1056 80 PCR rxns $ 490 Linear expression cassettes allow researchers to transfect cells on the same day that they receive the product. The results can be observed as early as the next day. No cloning or sequencing steps are required. This unique linear format is suitable for high throughput experiments, and for conjugating tracers or carriers to the cassette. Does not include p53 RNAi positive control, PCR mastermix, or transfection reagent AvantGene. Catalog Reference Amount List Price Cat # ABP-LS-1025 40 PCR rxns $ 190 Cat # ABP-LS-1026 80 PCR rxns $ 290 SilenCircle RNAi Transcription Complete Kit SilenCircle is a ready-to-ligate DNA vector that enables a permanent RNAi effect through drug selection. Compared to other vectors in the market, our special design precisely generates RNA molecules of standard size without introducing extra bases, thus chance of success is maximized. The complete kit includes a p53 RNAi positive control, T4 DNA Ligase, and AvantGene transfection reagent. Catalog Reference Amount List Price Cat # ABP-SC-1075 5 ligation rxns $290 Cat # ABP-SC-1076 10 ligation rxns $490
SilenCircle RNAi Transcription Kit
SilenCircle is a ready-to-ligate DNA vector that enables a permanent RNAi effect through drug selection. Compared to other vectors in the market, our special design precisely generates RNA molecules of standard size without introducing extra bases, thus chance of success is maximized. This does not include a p53 RNAi positive control, T4 DNA Ligase, or AvantGene transfection reagent.
Catalog Reference Amount List Price Cat # ABP-SC-1030 5 ligation rxns $ 190 Cat # ABP-SC-1031 10 ligation rxns $ 290 AvantGene Transfection Reagent
AvantGene is a broad range transfection reagent that efficiently delivers DNA molecules into a variety of cultured cells. AvantGene has been extensively tested by Allele scientists in Hela, 293, 293T, CHO, COS-7, HUVEC, 3T3-L1, NIH 3T3, MCF-7, Vero E6, C2C12 and rat L6 muscle cells and found to be superior to many other transfection reagents. Catalog Reference Amount List Price Cat # ABP-GR-2099 0.125ml (50 rxns) $ 80 Cat # ABP-GR-2100 0.25ml (100 rxns) $ 135 AvantGeneSi Transfection Reagent
AvantGeneSi is a specialized transfection reagent that efficiently delivers siRNA molecules into a variety of cultured cells including Hela, 293, 293T, CHO, COS-7, HUVEC, 3T3-L1, NIH 3T3, MCF-7, Vero E6, C2C12 cells. AvantGeneSi has been extensively tested by Allele scientists for the special purpose of RNAi-related transfections and found to be superior to many other transfection reagents. AvantGeneSi can also be used for transfection of other RNA and DNA oligos. Catalog Reference Amount List Price Cat # ABP-GR-2109 0.125ml (50 rxns) $ 81 Cat # ABP-GR-2110 0.25ml (100 rxns) $ 145 High Concentration T4 DNA Ligase (10 units/µl) Purified from an E.coli strain that carries a plasmid harboring the coding sequence of this enzyme. High quality and very competitive price comparing with the same product from other vendors. Catalog Reference Amount List Price Cat # ABP-SC-2075 100 units $ 30 Cat # ABP-SC-2076 500 units $ 119
T4 Polynucleotide Kinase (5 units/µl)
Purified from recombinant E. coli. Ideal for kinase reaction by adding g-phosphate from ATP to the 5' terminus of polynucleotides with 5' hydroxyl group. Catalog Reference Amount List Price Cat # ABP-RD-2015 100 units $ 35 Cat # ABP-RD-2016 1000 units $ 299
Taq DNA Polymerase (5 units/µl)
Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a double strand specific 5' to 3' exonuclease activity. Catalog Reference Amount List Price Cat # ABP-GR-2000 100 units $ 19 Cat # ABP-GR-2001 500 units $ 79 Cat # ABP-GR-2002 1000 units $ 125 High Efficiency Competent Cells SC33
Ideal for generation of high-difficulty construct and propagation of large-size or low-concentration plasmid. SC33 HE Competent Cells consistently provides very high transformation efficiency: 1x1010 cfu/µg of pUC19 plasmid DNA. The strain is more stable in maintaining low level of recombination than other commonly used commercial competent cells. Catalog Reference Amount List Price Cat # ABP-SC-2300 05 X 60µl $ 40 Cat # ABP-SC-2302 20 X 60µl $ 135 Cat # ABP-SC-2305 50 X 60µl $ 295 Cat # ABP-SC-2310 96 x 60µl $ 525
Highly purified yeast tRNA as a carrier for precipitation of small amount of nucleic acids or as a blocking agent in hybridization or binding reactions. Catalog Reference Amount List Price Cat # ABP-GR-2005 200 µg $ 49 Clean-Cut Protease (5 units/µl)
A viral 3C family protease, independently isolated by Allele scientists from a novel viral strain. The enzyme specifically recognizes a cleavage site of ELWSQ/X (X can be any small amino acid). Insert the coding sequence of this recognition site between your cDNA regions encoding two protein domains, e.g. a functional protein and a purification tag, during cloning. Produce fusion protein, followed by a room temperature protease reaction of 1-2 hours in virtually any buffer. If desired, the protease may be removed. Catalog Reference Amount List Price Cat # ABP-GR-2055 500 units $ 199
Pre-Made Agar Plates More antibiotics, dyes, growth supplements or their combinations than those listed below are available to meet special needs. The plates are dated and guaranteed for 2 months from the date of shipment although many antibiotic plates can be stored at 40C for a longer time.
Cat# Product Description Plate Size Packaging Price SC-2310 Lauria-Bertani (LB) Agar 100 x 15 mm 10 plates/pack $15.00 SC-2311 LB Agar, 50 mg/ml Ampicillin 100 x 15 mm 10 plates/pack $15.00 SC-2312 LB Agar, 100 mg/ml Ampicillin 100 x 15 mm 10 plates/pack $15.00 SC-2313 LB Agar, 50 mg/ml Amp., 0.1 mM IPTG, 60 mg/ml X-gal 100 x 15 mm 10 plates/pack $18.00 SC-2314 LB Agar, 75 mg/ml Carbenicillin 100 x 15 mm 10 plates/pack $17.00 SC-2315 LB Agar, 34 mg/ml Chloramphenicol 100 x 15 mm 10 plates/pack $17.00 SC-2316 LB Agar, 50 mg/ml Kanamycin 100 x 15 mm 10 plates/pack $17.00 SC-2317 LB Agar, 35 mg/ml Tetracycline 100 x 15 mm 10 plates/pack $17.00 SC-2318 Agar plates to your specifications 100 x 15 mm 10 plates/pack Inquire SC-2319 Agar plates to your specifications 150 x 15 mm 10 plates/pack Inquire
Products in the Pipeline:
-based on a novel nuclease protection technology, truly quantitative, easy to use!
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