- Allele Biotech High Throughput Plate Oligos

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Allele Oligo Service now offers plate oligos at new low prices. Every oligos is strictly controlled for quality, suitable for gene synthesis, chip printing, genotyping, and more. If you have enjoyed our quick turnaround next day oligo service, try our plate oligos and you will find it convenient and high quality.
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- Newly Introduced Modifications

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Triple-Labeled Probes for Internally Controlled Real-Time PCR
5´ FAM-Cy5.5--target sequence specific probe--Dabcyl3´ 100 nmol $450 + $1.00/base
5´ FAM--control target sequence probe--Dabcyl3´ 100 nmol $250 + $1.00/base
The internal positive control is supplied by a plasmid encompassing a sequence similar to the assay target sequence except for the probe hybridization site. The multiplex qPCR is performed with both probes and one set of outside amplification primers. The PCR products are monitored by using a single excitation source at 470 nm (laser of LED) and emissions at 530 nm from FAM, indicating control product, or 705 nm from Cy5.5 by FRET from FAM, indicating assay product.
FRET-Taqman: Jothikumar et al. 2009 BioTechniques Vol 46, P519-524
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- Same Day Oligo Prices at Starting Scale of 50 nmol
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Compare these new prices with other suppliers’, you will notice that Allele oligos are offered for next day delivery, synthesized on larger scales (50 nmol vs 10 or 25 nmol), and STILL at prices equal to or lower than competitors! Long oligos are our specialty, when others refuse your orders because they can’t make 100mers or longer, we can.
Allele offers discounted pricing for all academic institutions, please email us at oligo@allelebiotech.com for specifics.
- No setup fee, free desalting, fully deprotected and ready to use, conveniently delivered in ddH2O with free concentration normalization.
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- Modified Oligo Prices at 50 or 200 nmol Scales (next day delivery for 3’ modified oligos)
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Same price for 50 or 200 nmol scales. Compare these prices to $60 - $100 for the same products from other major oligo suppliers. Order now and stop paying unnecessary fees to use oligos with these common modifications. Allele can offer these better prices because we use our own oligo synthesis chemicals for many of the modifications and are pleased to pass the lower prices to our customers.
More options are provided based on customer request, if you need to have modifications not listed in the table (JOE, HEX, etc), please email oligo@allelebiotech.com
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- Purification Price
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The prices apply to 0.05 or 0.20 umol.
Even with advanced technology, high-performance machines and elaborate programs, the efficiency of each chemical step of oligo synthesis is seldom above 99%. As a result, deletion events at 1-2% of elongating oligos must occur at each and every position. As the oligo length increases, these deletion mutations accumulate; therefore, long oligos are more affected by this problem than shorter oligos. We strongly recommend PAGE purification for oligonucleotides greater than 45 bases in length. We cannot guarantee the quality of oligonucleotides longer than 45 bases in length unless they are purified.
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