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***General***

 

        The protein expression custom service team of Allele Biotech, now including Orbigen, provides services at all stages of the protein expression, production, and purification processes from gene cloning to large-scale fermentation. More than a decade ago, our technical staff offered the first commercial Baculovirus protein expression services to address researchers' unmet gene expression needs. Today we are on the cutting edge of a variety of expression and antibody production technologies so that you can accelerate your research by taking advantage of our comprehensive range of services.

 

Choice of Protein Expression Services

 

        Whether you need fast and economical bacterial expression or mammalian proteins with specific posttranslational modifications, we have the right service for you (see table below or Left Navigator)

 

Table. Choose Your Expression Systems
Host System Details Advantages Disadvantages
T7 (pET)
Economical. Simple culture conditions. Easy scale up.
Poor protein solubility. Low yield for many eukaryotic proteins.
Baculovirus
Near mammalian post-translational modifications. Much higher yield than mammalian systems.
Demanding media requirements.
CMV
Highest level of protein processing.
Demanding media requirements. Low protein yield.
Mamalian
Retrovirus
Wide selection of expression host cells. Mammalian processing.
Lengthy procedure. Demanding media requirements.

 

 

Modularized Service to Fit Your Needs

 

        With Allele Biotech's custom service program, everything from subcloning to media optimization is available for your precise requirements. Backed by years of experience, and cutting-edge technology, Orbigen's custom service staff will work with you to make sure you get the quality you need for the results you desire.

 

Table. Comprehensive Protein Expression offerings
Service
Bacteria
Yeast
Insect
Mammalian
Subclone gene into expression vector
Transfect/transform
Propagate cells
Prepare & titer viral stores
Optimize host strain
Optimize media
Optimize growth and expression conditions
Harvest & prepare cell lysates
Protein Purification (6xHis and/or GST-tagged proteins only)
Protein characterization

 

 

Flexible Scale

 

        Depending on your research needs and your familiarity with the expression profile of your proteins of interest, you may choose from a range of expression scales.

 

Table. Protein Production Scales Offered
Bacteria
Insect
Mammalian
1-50 L
1-100 L
1-10 L

 

 

 

We also provide Gene Synthesis service if you do not have the cDNA cloned in appropriate expression vectors >>More Details

Allele Biotechnology and Pharmaceuticals
9924 Mesa Rim Road San Diego, CA 92121
Tel: (800) 991-RNAi (858) 587-6645  Fax: (858) 587-6692
oligo@allelebiotech.com