Our PhoenixTM Retroviral Expression System offers the most time-tested method for producing infectious, replication-incompetent retrovirus that can efficiently transfer genes into a variety of mammalian cell types in vitro or in vivo. The PhoenixTM system is currently employed in over 2,500 laboratories worldwide. It is based on Moloney Murine Leukemia Virus (MMULV) and allows for delivery of genes to most dividing mammalian cell types, including many difficult-to-transfect cells. Now, we provide professional retrovirus service useing our Phoenix?Retroviral Expression system.
Available Services are as follows, depending on your research needs, you can choose any starting phase or combinations
Subcloning into Retrovirus Vector
Cat#
ABP-CS-MBRV001
Description:
In the case of gene expression, amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into Phoenix retroviral vector (provided by Allele Biotech, now including Orbigen). In the case of shRNA expression, design the target shRNA, oligo synthesis, then insert into Phoenix retroviral vector.
Verification of the subcloned gene by restriction digest and sequencing. Preparation of the recombinant vector DNA. In the case of gene expression, 5 µg of plasmid containing gene of interest plus complete vector sequence in electronic format. In the case of shRNA expression, detailed information about the gene of interest.
Required Materials:
Basic information
Deliverables:
1) 10 µg of sequecing verified plasmid containing the shRNA expression cassete 2) Final sequencing data 3) Detailed research report
Duration:
2 weeks
Price (USD):
$999
Package of Retrovirus
Cat#
ABP-CS-MBRV002
Description:
We also provide serive to help you package retrovirus particles using our popular and easy-to-use Phoenix Retrovirus System. More details, check here.
Required Materials:
5 µg of retrovirus vector containing shRNA expression cassete.
Deliverables:
1) High titer retrovirus solution 2) Detailed research report
