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Baculovirus Expression System
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Allele Biotech (now including Orbigen) Custom Baculovirus Protein Expression Service offers the fastest and most cost-effective way for you to take advantage of the Baculovirus expression technology.
- Features of Allele Baculovirus Protein Expression:
 Up to 6-fold yield enhancement and fast turnaround time- Experienced technical staff with more than 15 years of experience
- Modularized and customized service; Competitive price to fit your budget
- Available Services are as follows:
- Depending on your research needs, you can choose any starting phase or combinations
- Typical services:
 Baculovirus Expression Full Service (ABP-CS-PEBVFS1) Phase II, III and IV for $3800- Baculovirus Expression Basic Service (ABP-CS-PEBVBS1) Phase II for $1750
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- Phase I: Subcloning into Recombinant Baculovirus Transfer Vector
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| Cat# |
ABP-CS-PEBV001 |
| Description: |
 Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into recombinant Baculovirus transfer vector.
Verification of the subcloned gene by restriction digest and sequencing.
Preparation of the recombinant vector DNA suitable for co-transfection with baculovirus DNA.
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| Required Materials: |
5 ug of plasmid containing gene of interest plus complete vector sequence in electronic format. |
| Deliverables: |
1) 20 ug of ready-to-use recombinant Baculovirus transfer plasmid containing the gene of interest
2) Final sequencing data
3) Detailed research report |
| Duration: |
2 weeks: If insert > 2kb, one week additional per 1kb |
| Price (USD): |
$999: If insert > 2kb, $399 additional per 1kb; |
- Phase II: Generation and Evaluation of Baculovirus Recombinants
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| Cat# |
ABP-CS-PEBV002 |
| Description: |
Cotransfection of recombinant Baculovirus transfer vector containing gene of interest with 3 different linearized Baculovirus DNAs. Recombinant clones will be identified and amplified. The customer will choose the best recombinant baculovirus for the protein expression. |
| Required Materials: |
20 ug of ready to use recombinant Baculovirus transfer plasmid containing the gene of interest. |
| Deliverables: |
1) 3 cell pellets for evaluation of Baculovirus to be used and 50ml recombinant baculovirus solution
2) Detailed research report |
| Duration: |
2-3 weeks |
| Price (USD): |
$1750 |
- Phase III: Amplification of Recombinant Baculovirus Stock
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| Cat# |
ABP-CS-PEBV003 |
| Description: |
Amplification of the chosen recombinant Baculovirus and titration of final high-titer stock by end point dilution |
| Required Materials: |
At least 5 ml of recombinant Baculovirus stock |
| Deliverables: |
1) Specified quantity (in ml) of high-titer recombinant Baculovirus stock solution ready to express the protein of interest
2) Detailed research report |
| Duration: |
2-3 weeks |
| Price (USD): |
$599 for each 500 ml |
- Phase IV: Baculovirus Expression Optimization Service
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| Cat# |
ABP-CS-PEBV004 |
| Description: |
Amplification of the chosen recombinant Baculovirus to a high-titer stock of more than 1 x 108 pfu per ml
Infection of three insect cell lines (Sf21, Sf9, and T.ni using the high-titer stock and with or without booster virus
Cells are harvested at two different time points(72hr or 96hr) |
| Required Materials: |
At least 5-10 ml of recombinant Baculovirus stock. |
| Deliverables: |
1) 18 sets of cell pellets and media for evaluation of protein yield
2) Detailed research report |
| Duration: |
2-3 days |
| Price (USD): |
$1850 |
- Phase V: Protein Production Service
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| Cat# |
ABP-CS-PEBV005 |
| Description: |
Amplification of recombinant Baculovirus stock for protein production;
Expression of recombinant protein under optimized conditions. |
| Required Materials: |
At least 100 ml of high-titer (>108 pfu) recombinant Baculovirus stock. Optimized protein expression conditions are determined by previous evaluation project. |
| Deliverables: |
1) Cell pellets ready for protein purification or in the case of a secreted recombinant protein, the customer will receive the supernatant
2) Detailed research report |
| Duration: |
2~3 weeks (1~5 Liters)
4~5 weeks (5~20 Liters)
8~10 weeks (20 and above Liters) |
| Price (USD): |
$599 for each liter of culture |
- Phase VI: Protein Extraction & Purification
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| Cat# |
ABP-CS-PEBV006 |
| Description: |
Cell pellet harvested from 1 liter of insect culture will be lysed with our optimized reagents and protocol.
Protein purification using appropriate affinity resin for 6xHis or GST-tagged recombinant proteins.
Purification can be performed either under native or denatured conditions (as desired by the customer).
SDS Page and Western Blot will be performed for quality testing. |
| Required Materials: |
Cell pellet ready for extraction |
| Deliverables: |
1) Protein solution in standard or customer-specified buffer
2) SDS-PAGE or Western Blot data
3) Detailed research report |
| Duration: |
2-3 weeks |
| Price (USD): |
$1500/L, inquire the price for larger volume |
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