Allele Biotech, now including Orbigen, provides services at all stages of the protein expression, production, and purification processes from gene cloning to large-scale fermentation.
More than a decade ago, our technical staff offered the first commercial Baculovirus protein expression services to address researchers´ unmet gene expression needs. Today we are on the cutting edge of a variety of expression and antibody production technologies so that you can accelerate your research by taking advantage of our comprehensive range of services.
In terms of protein expression, bacteria and yeast are commonly used to express a variety of proteins because they are more convenient and less costly than other systems. However, they may not be suitable for producing mammalian proteins with important post-translation modifications (PTM). Insect cells can be used effectively for producing large quantities of mammalian proteins rather easily through baculovirus such as Allele´s Sapphire system. PTM in insect cells is not exactly the same as in mammalian cells, e.g. different glycosylation patterns. Mammalian cells are used for proteins that require appropriate PTM or are not soluble in other systems through either transient transfection or stable cell line establishment. Allele Biotech is developing its own system to use baculovirus for expression in mammalian cells that will avoid high costs associated with large scale transient transfection, or the time consuming stable cell line establishment.
Choice of Protein Expression Services
Whether you need fast and economical bacterial expression or mammalian proteins with specific posttranslational modifications, we have the right service for you
Host
System
Advantage
Disadvantage
Bacterial
T7
Economical Simple culture conditions Easy scale up
Poor protein solubility Low yield for many eukaryotic proteins
Yeast
Insect
Baculovirus
Near mammalian post-translational modifications Much higher yield than mammalian systems
Demanding media requirements
Mamalian
CMV
Highest level of protein processing
Demanding media requirements Low protein yield
Mamalian
Retrovirus
Wide selection of expression host cells Mammalian processing
Lengthy procedure Demanding media requirements
Modularized Service to Fit Your Needs
With Allele Biotech's custom service program, everything from subcloning to media optimization is available for your precise requirements. Backed by years of experience, and cutting-edge technology, Allele Biotech's custom service staff will work with you to make sure you get the quality you need for the results you desire.
Service
Bacteria
Yeast
Insect
Mammalian
Subclone gene into expression vector
Transfect/transform
Propagate cells
Prepare & titer viral stores
Optimize host strain
Optimize media
Optimize growth and expression conditions
Harvest & prepare cell lysates
Protein Purification (6xHis and/or GST-tagged proteins only)
Protein characterization
Flexible Scale
Depending on your research needs and your familiarity with the expression profile of your proteins of interest, you may choose from a range of expression scales.
Bacteria
Yeast
Insect
Mammalian
1-50L
1-50L
1-100L
1-10L
We also provide Gene Synthesis Service, if you do not have the appropriate cDNA sequence for target gene.
