mRNA expression has gained a great deal of recognition recently as a means for exogenous expression in mamallian cells. Through recent developments (Warren et al. 2010) mRNA can now be used to exogenously express functional enzymes, fluorescent markers, and various factors in cells over long periods of time without triggering immunoresponses.
Custom mRNA Template Service
Allele Biotech's custom mRNA services allow you to express your constructs using this ground breaking method without the hassles and troubleshooting associated with creating your own template. All we need is your construct (or your sequence), and your specifications, from there we can create a PCR template that will be sequence confirmed and guaranteed to produce your desired mRNA via in vitro transcription (IVT). Additionally, we can use this template to produce a specified amount of mRNA.
- Linear DNA templates are ready to use for IVT reactions.
- Coding sequence for a protein is flanked by 5’ and 3’ untranslated regions designed to maximize the translational efficiency and cytoplasmic stability of the mRNA.
- Templates drive incorporation of 120-nt polyA tails into RNA transcripts, eliminating the need to enzymatically polyadenylate IVT products before use.
- Additional specifications such as binding sites can be incorporated into template design.
Required Materials: Construct or sequence of insert.
Deliverables: 10µg of template, or a specified amount of mRNA (modified or unmodified).
Duration: Typically 2 - 4 weeks.
Price (USD): $3,000 for template construction, does not include gene synthesis; bulk pricing also available. Please contact us to discuss your project and receive a quote, email us at email@example.com or call us at 858-587-6645.