mRNA expression has gained a great deal of recognition recently as a means for exogenous expression in mamallian cells. Through recent developments (Warren et al. 2010) mRNA can now be used to exogenously express functional enzymes, fluorescent markers, and various factors in cells over long periods of time without triggering immunoresponses.
Allele Biotech's custom mRNA services allow you to express your constructs using this ground breaking method without the hassles and troubleshooting associated with creating your own template. All we need is your construct (or your sequence), and your specifications, from there we can create a PCR template that will be sequence confirmed and guaranteed to produce your desired mRNA via in vitro transcription (IVT). Additionally, we can use this template to produce a specified amount of mRNA.
Required Materials: Construct or sequence of insert.
Deliverables: 10µg of template, or a specified amount of mRNA (modified or unmodified).
Duration: Typically 2 - 4 weeks.
Price (USD): $3,000 for template construction, does not include gene synthesis; bulk pricing also available. Please contact us to discuss your project and receive a quote, email us at firstname.lastname@example.org or call us at 858-587-6645.