Genome editing is the process of editing an organisms DNA by altering, removing or adding nucleotides to the genome. This process is accomplished through the use of engineered nucleases that can make a double stranded “cut” or a single stranded “nick” in an organism’s DNA. These sites are altered and then repaired by homologous recombination or non-homologous end joining. This type of genetic engineering is useful for a number of applications, including targeted gene mutations, chromosomal rearrangement, and the creation of transgenic animals.
For a number of years Allele Biotechnology has focused on cellular reprogramming, specifically the ability to reprogram cells endogenously. This was achieved in 2012 with the publication of “Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA” in Scientific Reports. The next logical use for the powerful mRNA platform we developed was genome modification. Because of its high efficiency and the ability to use interchangeable small guiding RNA, we settled on CRISPR technology.
Our CRISPR system is completely based on validated modified messenger RNA (mRNA) technology. This approach increases CRISPR efficiency while reducing off target effects when combined with our highly precise sgRNA design. It allows for any cell type to be used as starting material, including induced pluripotent stem cells reprogrammed using our RNA platform. The resulting cell line is validated using deep sequencing to ensure only the desired changes were made.
The combination of cellular reprogramming and genome editing can serve as a powerful tool for developing assays and conducting novel research. To take advantage of this we offer both a custom service and reagents. Please fill out our contact form and someone from our technical staff will contact you to discuss your custom project. We can start with a number of different cell types and your project can start with cellular reprogramming to iPSCs, or proceed directly to genome modification. To view our reagent options proceed to the bottom of the page.