GFP-nAb

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What is GFP-nAb?

Optimized by the research team at Allele Biotechnology, GFP-nAb™ is a highly specific GFP (Green Fluorescent Protein) binding protein derived from camelids. It is characterized by a small barrel shaped structure (13 KDa, 2.5nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2M NaCl or 0.5% SDS). Experiments have shown that one molecule of GFP-nAb™ binds one molecule of GFP with a dissociation constant (Kd) in the sub nanomolar range. This makes GFP-nAb™ the ideal candidate for a variety of biological assays, some of which are listed below:

  • Immunoprecipitation / CO-IP
  • Quantitative analysis
  • Chromatin Immunoprecipitation (ChIP)
  • Superresolution Imaging

GFP-nAb™ binds a large number of commonly used fluorescent proteins derived from the original Aequorea victoria GFP, including EGFP, Venus, Cerulean, and EBFP2 (click here for a full list). Conveniently, it displays no binding affinity to non-jellyfish-derived fluorescent proteins, including the "mFruits" such as mCherry, or Allele's mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The GFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions and allow you to perform multiple immunoprecipiation reactions in a matter of minutes.

gfp-nab-gel.jpg

Complete Pulldown using the GFP-nAb™ Spin Kit.

EGFP-expressing Sf9 (insect) cell lysate contained a total of 16µg of EGFP in total volume of 500µl, determined spectrophotometrically. Following the GFP-nAb™ Spin Kit binding and wash protocols, the protein was eluted in 2 x 50 µl elution buffer (0.2M glycine pH 2.5), pooled, and neutralized with 10µl of 1M Tris base. Equal volumes of lysate input (I), flow-through (FT) after binding to GFP-nAb™ agarose resin, and elution (E) fractions were analyzed by SDS-PAGE followed by Coomassie staining. In this experiment, EGFP was quantitatively removed from the lysate.

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  • GFP-nAb Magnetic Agarose Kit (20 Reactions)

    [[20 Rxns,ABP-NAB-GFPXK20]] The GFP-nAb Magnetic Agarose Kit allows for quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ magnetic slurry binds a minimum of 0.5µg of GFP.  This kit contains all the...

    $469.00
    Add to Cart
  • GFP-nAb™ Magnetic Kit (20 Reactions)

    [[20 Rxns,ABP-NAB-GFPMK20]] The GFP-nAb™ Magnetic Kit allows for quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ magnetic slurry binds a minimum of 0.5µg of GFP.  This kit contains all the...

    $469.00
    Add to Cart
  • GFP-nAb Kit

    GFP-nAb™ Agarose Spin Kit (20 Reactions)

    [[20 Rxns,ABP-NAB-GFPAK20]] The GFP-nAb™ Agarose Spin Kit allows for quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP.  This kit contains all the...

    $469.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 0.25ml

    [[250µl,ABP-NAB-GFPX025]]   GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP...

    $219.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 0.5ml

    [[500µl,ABP-NAB-GFPX050]]   GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP...

    $399.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 1ml

    [[1ml,ABP-NAB-GFPX100]]   GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP...

    $719.00
    Add to Cart
  • GFP-nAb™ Agarose 0.25ml

    GFP-nAb™ Agarose 0.25ml

    [[250µl,ABP-NAB-GFPA025]] GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the ideal...

    $219.00
    Add to Cart
  • GFP-nAb

    GFP-nAb™ Agarose 0.5ml

    [[500µl,ABP-NAB-GFPA050]]   GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the...

    $399.00
    Add to Cart
  • GFP-nAb™ Agarose 1ml

    GFP-nAb™ Agarose 1ml

    [[1ml,ABP-NAB-GFPA100]] GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the ideal candidate for...

    $719.00
    Add to Cart
  • GFP-nAb Magnetic 0.5ml

    [[500µl,ABP-NAB-GFPM050]] GFP-nAb™ Magnetic beads allow for quick and convenient pulldown of GFP from solution with the use of a magnetic stand. GFP-nAb™ will quickly aggregate toward your magnet allowing for clean and complete...

    $399.00
    Add to Cart
  • GFP-nAb Magnetic 1ml

    [[1ml,ABP-NAB-GFPM100]] GFP-nAb™ Magnetic beads allow for quick and convenient pulldown of GFP from solution with the use of a magnetic stand. GFP-nAb™ will quickly aggregate toward your magnet allowing for clean and complete elution...

    $719.00
    Add to Cart
  • GFP-nAb Purified Protein

    [[25µg,ABP-NAB-GFPAB]] GFP-nAb™ purified protein is a single domain VHH antibody that binds GFP. It is supplied in the amount of 25µg at a 1mg/ml concentration. Components 25µg GFP-nAb™ Purified Protein 1mg/ml...

    $199.00
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