GFP-nAb

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What is GFP-nAb?

Optimized by the research team at Allele Biotechnology, GFP-nAb™ is a highly specific GFP (Green Fluorescent Protein) binding protein derived from camelids. It is characterized by a small barrel shaped structure (13 KDa, 2.5nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2M NaCl or 0.5% SDS). Experiments have shown that one molecule of GFP-nAb™ binds one molecule of GFP with a dissociation constant (Kd) in the sub nanomolar range. This makes GFP-nAb™ the ideal candidate for a variety of biological assays, some of which are listed below:

  • Immunoprecipitation / CO-IP
  • Quantitative analysis
  • Chromatin Immunoprecipitation (ChIP)
  • Superresolution Imaging

GFP-nAb™ binds a large number of commonly used fluorescent proteins derived from the original Aequorea victoria GFP, including EGFP, Venus, Cerulean, and EBFP2 (click here for a full list). Conveniently, it displays no binding affinity to non-jellyfish-derived fluorescent proteins, including the "mFruits" such as mCherry, or Allele's mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The GFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions and allow you to perform multiple immunoprecipiation reactions in a matter of minutes.

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Complete Pulldown using the GFP-nAb™ Spin Kit.

EGFP-expressing Sf9 (insect) cell lysate contained a total of 16µg of EGFP in total volume of 500µl, determined spectrophotometrically. Following the GFP-nAb™ Spin Kit binding and wash protocols, the protein was eluted in 2 x 50 µl elution buffer (0.2M glycine pH 2.5), pooled, and neutralized with 10µl of 1M Tris base. Equal volumes of lysate input (I), flow-through (FT) after binding to GFP-nAb™ agarose resin, and elution (E) fractions were analyzed by SDS-PAGE followed by Coomassie staining. In this experiment, EGFP was quantitatively removed from the lysate.

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  • GFP-nAb™ Magnetic 0.25ml

    [[250µl,ABP-NAB-GFPM025]] GFP-nAb™ Magnetic beads allow for quick and convenient pulldown of GFP from solution with the use of a magnetic stand. GFP-nAb™ will quickly aggregate toward your magnet allowing for clean and complete...

    $219.00
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  • nAb Spin Kit Columns and Buffers

    [[20 Reactions,ABP-NAB-SPINK20]] A convenient kit consisting of 20 spin columns and all the required buffers for our nAb line of products.  The nAb Spin Kit Columns and Buffers can be used with our nAb controls or to supplement your existing nAb...

    $99.00
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  • nAb Agarose Control 0.5ml

    [[500µl,ABP-nAb-ACNTRL5]] Allele Biotech’s Agarose Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using agarose support. The blocked resin is also useful as a negative...

    $49.00
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  • nAb Magnetic Agarose Control 0.5ml

    [[500µl,ABP-nAb-XCNTRL5]] Allele Biotech’s Magnetic Agarose Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using agarose support. The blocked resin is also useful as a...

    $49.00
    Add to Cart
  • nAb Magnetic Control 0.5ml

    [[500µl,ABP-nAb-MCNTRL5]] Allele Biotech’s Magnetic Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using magnetic support. The blocked resin is also useful as a negative...

    $49.00
    Add to Cart