In Situ hybridization is a technique to detect the expression pattern of a particular gene sequence in cells or tissues using a complimentary sequence known as a probe. The probes are typically labeled with a fluorochrome or an enzyme so they can be detected either by using a fluorescent microscope or a color substrate which is visible by brightfield microscope.
The colorimetric In Situ hybridization we use here at Allele Biotech is a quantitative, highly sensitive technique that can detect one single gene in a cell by highlighting it as a colored dot. These dots can then be counted by the researcher or by image analysis software to get numerical data.
Please contact us using the form below to speak with a scientist or obtain a quote for our in situ hybridization services.
We offer the following ISH services:
- DNA In situ
- mRNA in situ using riboprobes
- TUNEL Assay