In situ hybridization - ISH

In Situ hybridization is a technique to detect the expression pattern of a particular gene sequence in cells or tissues using a complimentary sequence known as a probe. The probes are typically labeled with a fluorochrome or an enzyme so they can be detected either by using a fluorescent microscope or a color substrate which is visible by brightfield microscope.

The colorimetric In Situ hybridization we use here at Allele Biotech is a quantitative, highly sensitive technique that can detect one single gene in a cell by highlighting it as a colored dot. These dots can then be counted by the researcher or by image analysis software to get numerical data.

Please contact us using the form below to speak with a scientist or obtain a quote for our in situ hybridization services.

We offer the following ISH services:

  • DNA In situ
  • mRNA in situ using riboprobes
  • RNAscope
  • TUNEL Assay

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