mNeonGreen

For a span of 20 years, researchers have by and large looked to Aequorea victoria Green Fluorescent protein (GFP) for their imaging, and tracking needs. Over this time period, the development of GFP has elicited a wide spectrum of colors and optimized versions for a multitude of applications. As imaging moves into the super resolution era, most common fluorescent proteins, including GFP, may not possess certain characteristics beneficial for super resolution applications. With this in mind, Allele now offers a new high performance monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein to date, and is an excellent fusion tag for traditional imaging as well as stochastic single-molecule super resolution imaging. Lastly it is a stellar fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent proteins.  For more information on mNeonGreen's properties view our publication, "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum" in the journal Nature MethodsClick here for Licensing opportunities, click here for our Active License List

 

Allele Biotechnology now has a portal for information on various super resolution imaging techniques (PALM, STORM, SIM, STED), tools, and equipment.  To access this super resolution imaging portal click here

 

Fig. 1 mNeonGreen absorbance, excitation, and emission                                                                                                                      
em-ex-graph.png
Fig. 2 Basic Properties

mNeonGreen

 

Excitation Max (nm)

506

Emission Max (nm)

517

Quantum Yield

0.8

Extinction coefficient

116,000

Brightness

92.8

pKa

5.7

Fig. 3 Super Resolution Imaging Localization Data

Fluorescent

Protein

# Molecules Localized

# Photons per Molecule

PSF Width (nm)

mNeonGreen

33,783

663 ± 259

167.3 ± 34.1

Clover

15,281

408 ± 263

176.4 ± 57.6

mEGFP

6,803

523 ± 261

165.8 ± 37.1

Fig. 4 Fluorescence imaging of mNeonGreen fusion vectors.(from left to right: actin, plasma membrane, microtubules, replication foci.)

actin-color.png

actin-binding-color.png

microtubules-color.png

replicationfoci-color.png 

 

Site License and Advanced User license have access to these mNeonGreen fusions and constructs carried by Allele Biotechnology.

 

mNeonGreen  
BASIC    
pNCS-mNeonGreen (E.coli Expression)
 
Fusions- From: Shaner, N.C. et al. "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature Methods 2013, ePub March 24.
mNeonGreen-Actin-7
mNeonGreen-alpha-Actinin-19
mNeonGreen-Annexin-12
mNeonGreen-C1                   
mNeonGreen-Clathrin-15         
mNeonGreen-Cx26-7               
mNeonGreen-Cx43-7               
mNeonGreen-EB3-7               
mNeonGreen-Endo-14 - RhoB-14
mNeonGreen-ER-5  
mNeonGreen-Farnesyl-5           
mNeonGreen-Golgi-7  - GalT-7
mNeonGreen-H2B-6
mNeonGreen-Lysosomes-20  - LAMP1-20
mNeonGreen-N1  
mNeonGreen-Paxillin-22
mNeonGreen-Tubulin-6
mNeonGreen-Vimentin-7
mNeonGreen-Zyxin-6
mNeonGreen-Actin-C-18
mNeonGreen-Actin-C-35
mNeonGreen-Beta-Catenin-20
mNeonGreen-CAF1-C-10           
mNeonGreen-Calnexin-N-14       
mNeonGreen-Calreticulin-N-16   
mNeonGreen-Caveolin-C-10       
mNeonGreen-CEBPA237-C-4         
mNeonGreen-Cortactin-C-12       
mNeonGreen-C-Src-7             
mNeonGreen-Fascin-C-10         
mNeonGreen-Fibrillarin-7
mNeonGreen-FilaminA-C-14
mNeonGreen-H2B-C-10
mNeonGreen-Keratin-17
mNeonGreen-LC3B-C-7
mNeonGreen-LC-Myosin-N-7
mNeonGreen-Lifeact-7
mNeonGreen-MannII-N-10  - MannosidaseII
mNeonGreen-MAPTau-C-10
mNeonGreen-MAPTau-N-10
mNeonGreen-MyosinIIA-N-14
mNeonGreen-Myotilin-C-14
mNeonGreen-Nup50-N-10
mNeonGreen-PCNA-19
mNeonGreen-PDHA1-N-10
mNeonGreen-Plastin-C-10
mNeonGreen-PMP-N-10
mNeonGreen-PTK2-N-10
mNeonGreen-Rab4a-7
mNeonGreen-Rab5a-7
mNeonGreen-SiT-N-15  - sialyltransferase
mNeonGreen-Talin-18
mNeonGreen-TFR-20
mNeonGreen-TOMM20-N-10
mNeonGreen-TPX2-N-10
mNeonGreen-Tubulin-C-18
mNeonGreen-Tubulin-C-35
mNeonGreen-VASP-N-10
mNeonGreen-VE-Cadherin-N-10
mNeonGreen-Vinculin-N-21
mNeonGreen-ZO1-C-14
 
Yeast Optimized mNeonGreen