Allele’s photoconvertible proteins allow researchers to track the changes in localization in living cells by changing from green to red. pmClavGR2-CT is a monomeric photoconvertible fluorescent expression vector with multiple cloning sites for fusions to the C terminus. Compared with previously available photoconvertible FPs, mClavGR2 has improved photostability of the red state under confocal illumination conditions, 3644 over mEOS2’s 2700 and Dendra2’s 2420. Most notable among other advantages of mClavGR2 is its monomeric structure, its highly optimized and rapid folding efficiency, and its superior photoconversion efficiency due to the high pKa of the green state. Licensing opportunities, Active Licence List
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mMaple is a monomeric green-to-red pcFP variant derived from mClavGR2. This pcFP possesses significant benefits over similar FPs for most imaging applications. The ablity to photoconvert from green-to-red and the high green state brightness allows mMaple to be utilized in (f-)PALM/STORM and SIM super-resolution microscopy techniques. Studies showed that mMaple constructs reliably yielded more protein localizations than mEos2 or mClavGR2 per cell (3.4× and 2.3×, respectively). Other advantages include high concentration of properly folded fusion proteins and elevated photostability.
Spectral characterization of mMaple
mMaple fusions expressed in mammalian cells