PhagoTest

 

Leukocyte Phagocytosis

A complete reagent kit for the quantitative determination of leukocyte phagocytosis in whole blood using Flow Cytometry. Phagocytosis is an important immunological function that allows cells to remove pathogens and debris. Fluorescently labeled probes are absorbed by cells and measured to characterize phagocytic activity.

 

Work Flow

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Indications

A number of genetic and acquired defects can significantly lower neutrophil activity in cells, including:

  • Actin dysfunction
  • Complement receptor deficiency
  • CD32 defect
  • Trauma
  • Diabetes
  • Renal failure
  • Infection

Advantages

  • A highly sensitive test that uses heparanized whole blood
  • Samples can be succesfully used up to 48 hours after collection with no lost and up to 72 hours after collection with minimal data loss
  • PhagoTest can be used with bovine, porcine, murine, canine and lapine blood samples
  • A complete kit containing all the necessary buffers and reagents for 20 or 100 patient samples

Publications

Athanasios Filias, Georgios L Theodorou, Sofia Mouzopoulou, Anastasia A Varvarigou, Stephanos Mantagos, Marina Karakantza; Phagocytic ability of neutrophils and monocytes in neonates;
BMC Pediatr. 2011; 11: 29. (PubMed)
 
 
Menge, Ch., Meufeld, B., Hirt, W., Schmeer, N., Bauerfeind, R., Baljer, G. & L.H. Wieler. 1998. Compensation of preliminary blood phagocyte immaturity in the newborn calf. Vet. Immunol. Immunopathol. 62: 309-321. (PubMed)
 
Werling D., Howard, C.J., Niederer, E., Straub, O.C., Saalmüller, A. & W. Langhans. 1998. Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus. Vet. Immunol. Immunopathol. 62: 185-195. (PubMed)
 
Fachinger, V., Schlapp, T., Strube, W., Schmeer, N. & A. Saalmüller. 2000. Poxvirus-induced immunostimulating effects on porcine leukocytes. J. Virol. 74: 7943-7951. (PubMed)
 
Seiler, P., Aichele, P., Raupach, B., Odermatt, B., Steinhoff, U. & S.H.E. Kaufman. 2000. Rapid neutrophil response controls fast-replicating intracellular bacteria but not slow-replicating Mycobacterium tuberculosis. J. Infect. Dis. 181: 671-80. (PubMed)

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