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What is RFP-nAb?

RFP-nAb™ is a highly specific RFP (Red Fluorescent Protein) binding protein derived from camelids. It is characterized by a small barrel shaped structure (13 KDa, 2.5nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2M NaCl or 0.5% SDS). RFP-nAb is an excellent choice for a variety of experiments and assays, including:

  • Immunoprecipitation / CO-IP
  • Quantitative analysis
  • Chromatin Immunoprecipitation (ChIP)
  • Superresolution Imaging

RFP-nAb™ binds a large number of commonly used fluorescent proteins derived from DsRed (Discosoma Red) commonly known as the mFruits, including mRFP, mCherry, mOrange, and mPlum. Conveniently, it displays no binding affinity to jellyfish-derived fluorescent proteins including GFP and its variants, or Allele's mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The RFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down RFP fusions. Each RFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions and allow you to perform multiple immunoprecipiation reactions in a matter of minutes.

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  • nAb Magnetic Agarose Control 0.5ml

    [[500µl,ABP-nAb-XCNTRL5]] Allele Biotech’s Magnetic Agarose Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using agarose support. The blocked resin is also useful as a...

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