Introduction
Alleleustrious pmTFP1-N is a mammalian expression vector with a multiple cloning site for fusions to the N terminus of mTFP1, also suitable for expression of mTFP1 alone in mammalian cells. The properties of Alleleustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins.
Source
Engineered variant of Clavularia sp. fluorescent protein cFP484
Recomended Use
To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended.
Features
* More than 3-fold brighter than ECFP
* More than twice as photostable as ECFP
* Single emission peak
* Single-exponential fluorescence lifetime ( = 3.2 ns)
* Can be co-imaged with red and yellow FPs
* Low sensitivity to acidic pH(fluorescence pKa=4.3)
* Mammalian expression vector ready to transfect your favorite cells
* True monomer that will not aggregate or cause non-specific interactions
Reconstitution
10 g provided in lyophilized powder form. Reconstitute with 10 L of nuclease-free water for a final concentration of 1 g/L.
Storage
Store at -80C for long-term preservation.
Plasmid Image AND MCS
References
Ai HW, Henderson JN, Remington SJ, Campbell RE.
Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization
and applications in fluorescence imaging. Biochem J. 2006 Jul 24
Shaner NC, Steinbach PA, Tsien RY.
A guide to choosing fluorescent proteins. Nat Methods. 2005 Dec;2(12):905-09
Synthetic mTFP1 cDNA
1 atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat
gaagatcaag
61 ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc
121
aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc
181
ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac
241 cccgacgaca
tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc
301 accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc
catggaggag
361
gactccttca tctacgagat acacctcaag ggcgagaact
tcccccccaa cggccccgtg
421 atgcagaaga agaccaccgg ctgggacgcc
tccaccgaga ggatgtacgt gcgcgacggc
481
gtgctgaagg gcgacgtcaa
gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt
541
gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg
601 gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag
661
agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagta a
Full sequence of vector is available at here 
.
IMPORTANT NOTICE TO PURCHASER
These products are the subjects of pending U.S. and foreign patents.
Academic or government institutions:
Orders may be placed by contacting Allele Biotech (oligo@allelebiotech.com / 858-587-6645) or registering online at www.allelebiotech.
com and placing an online order. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive,
royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits
the right to sell or otherwise transfer this product or its components to a third party. Any other use of this product will require a sublicensing
agreement from Allele Biotech. For sub-license information please contact us via phone or email.
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Institutions that wish to use this product in non-commercial or commercial applications are required to obtain a
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